Bordeaux mixture is used as preventive treatment against mildew, a parasitic fungus of vine, since the end of the 19th century. This fungicide is composed of copper sulphate. It is one of the only fungicides allowed in biological agriculture in small doses. High quantities used at the beginning of the 20th century induced an accumulation of copper in vineyards soils, particularly in Bordeaux region. Copper is an essential metal for plants but at high dose, it can have toxic effects. Nowadays, it is important to understand transfer mechanisms between soil and plant to prevent toxicity issues. One method to assess metal bioavailability in soil is the use of passive samplers such as DGT (Diffusive Gradients in Thin films). The objective of this study is to define copper contamination in Bordeaux vineyards and to validate DGT device as an indicator of copper bioavailability in soils. Ten vineyard’s soils have been chosen in Pessac Leognan appellation, and copper bioavailability was estimated in March and May and compared to metal concentration in vine leaves. Finally, DGT technique was compared to others measurements of copper bioavailability in soils.