Correlative radioimaging and mass spectrometry imaging: a powerful combination to study 14 C-graphene oxide in vivo biodistribution - Inrap - Institut national de recherches archéologiques préventives Accéder directement au contenu
Article Dans Une Revue Nanoscale Année : 2023

Correlative radioimaging and mass spectrometry imaging: a powerful combination to study 14 C-graphene oxide in vivo biodistribution

Hélène Cazier
  • Fonction : Auteur
Carole Malgorn
  • Fonction : Auteur
Dominique Georgin
  • Fonction : Auteur
Nathalie Fresneau
  • Fonction : Auteur
Fabrice Beau
  • Fonction : Auteur
Kostas Kostarelos
Cyrill Bussy
Stéphane Campidelli
Mathieu Pinault
  • Fonction : Auteur
Martine Mayne-L'Hermite
  • Fonction : Auteur
Frédéric Taran
Christophe Junot
  • Fonction : Auteur
Benoit Colsch
  • Fonction : Auteur

Résumé

Research on graphene based nanomaterials has flourished in the last decade due their unique properties and emerging socio-economic impact. In the context of their potential exploitation for biomedical applications, there is a growing need for the development of more efficient imaging techniques to track the fate of these materials. Herein we propose the first correlative imaging approach based on the combination of radioimaging and mass spectrometry imaging for the detection of Graphene Oxide (GO) labelled with carbon-14 in mice. In this study, 14C-graphene oxide nanoribbons were produced from the oxidative opening of 14C-carbon nanotubes, and were then intensively sonicated to provide nano-size 14C-GO flakes. After Intravenous administration in mice, 14C-GO distribution was quantified by radioimaging performed on tissue slices. On the same slices, MS-imaging provided a highly resolved distribution map of the nanomaterial based on the detection of specific radical anionic carbon clusters ranging from C2˙− to C9˙− with a base peak at m/z 72 (12C) and 74 (14C) under negative laser desorption ionization mass spectrometry (LDI-MS) conditions. This proof of concept approach synergizes the strength of each technique and could be advantageous in the pre-clinical development of future Graphene-based biomedical applications.
Fichier principal
Vignette du fichier
2023-Correlative radioimaging and mass spectrometry imaging- a powerful combination to study 14C-graphene oxide in vivo biodistribution.pdf (3.79 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-04465763 , version 1 (19-02-2024)

Identifiants

Citer

Hélène Cazier, Carole Malgorn, Dominique Georgin, Nathalie Fresneau, Fabrice Beau, et al.. Correlative radioimaging and mass spectrometry imaging: a powerful combination to study 14 C-graphene oxide in vivo biodistribution. Nanoscale, 2023, 15 (11), pp.5510-5518. ⟨10.1039/D2NR06753F⟩. ⟨hal-04465763⟩
19 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More